Composite

Part:BBa_K936013:Design

Designed by: Mattan Hamou   Group: iGEM12_UC_Davis   (2012-07-24)


pelB-LC-Cutinase fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal AgeI site found at 666
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Adding sequence of pelB in front of sequence for Cuntinase


Source

http://aem.asm.org/content/78/5/1556.long

Constructed from pelB leader sequence and protein coding sequence for LC-Cutinase

References